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July 28, 2016
Q. Can I use pH paper to confirm cleaning and rinsing of labware to comply with lab accreditation requirements?
A. If you are going to use pH strips to comply with lab accreditation requirements, you need to verify that your detergents are not interfering with the strips and papers. Detergents and surface active agents can interfere with some pH paper by causing a decrease of several pH units in reading. Please test any pH paper you intend to use with these detergents to determine if there is any interference before adapting this procedure for use with pH paper.
If deionized water is used as the sample water, a slight amount (10-20 mg/L) of reagent grade, non-buffering salt (NaCl, CaCl2) should be added to the sample water to allow pH meter to function properly. If you want to avoid contaminating clean glassware, dump the glassware testing solution into a triple rinsed beaker and then add the non-buffering salt prior to measuring the pH with a meter.
July 28, 2016
Q. We are looking to clean denatured proteins from our medical device along with baked-on desiccated blood that is not removed completely. Manual scraping removes the residual but is not acceptable. Need a cleaner that will dissolve all bio-matter. Cleaning process is to prepare device for autoclave sterilization.
A. To clean denatured proteins use a warm (not exceeding 130F / 55C) 1-2% Tergazyme® Enzyme-Active Powdered Detergent (1.25 – 2.5 oz/gal or 10 – 20 g/L) solution to soak, or ideally ultrasonically soak the substrates.
The detergent contains both powerful emulsifiers for organic and oily residue, along with proteases to greatly assist in protein removal.
Visually inspect and scrub as needed to speed up the cleaning process. Soak for at least 20 minutes. Higher solution concentrations increase the detergent’s capacity or the amount of residue that can be removed.
Be sure to use freshly made Tergazyme solutions that are less than 8 hours old. Older solutions of Tergazyme cleaner may lose some of their enzymatic detergency due to the enzyme digesting itself in solution once it is dissolved.
Similarly, temperatures above 130F/55C can degrade the enzyme and alter its effectiveness.
Rinse thoroughly to remove the detergent solution and residues after cleaning.
May 5, 2016
A Solution for Waste Disposal of Volatile Organic Compounds to Increase Standards of Health
A major concern in the field of chemical science is the proper care and disposal of hazardous wastes. With high-performance liquid chromatography (HPLC) machines, solvent wastes must be contained for disposal. However, these liquids are often volatile organic compounds (VOCs), meaning they will easily vaporize in the lab and spread through the air. In order to contain these vapors, which are potential health hazards for people working in the lab, improved waste containment must be implemented. Common lab practices have not been sufficient in containing these vapors. Continue reading